Dr. Nicholas Borotto

Biography

My research program strives to improve mass spectrometric-based detection and analysis of biomolecules. In particular, we pair mass spectrometry with chemical derivatization, photon irradiation, ion mobility, and radical chemistry to elucidate the three-dimensional structure of proteins, better characterize the acidic and hydrophobic proteome, detect and localize post-translational modifications. Centered at the interface of chemistry and biology, my research program provides students with the opportunity to tackle both biochemically-focused projects and biophysical questions at the core of the techniques themselves. Currently, my group is recruiting students for three projects:

1) Equipping a carbon monoxide laser to a mass spectrometer, characterizing the behavior of irradiated biomolecules, and applying infrared multiphoton dissociation (IRMPD) to instruments and at pressure regimes traditionally precluded from this technique.

2) Probing protein three-dimensional structure with photocaged small molecule reagents both in vitro and in vivo and demonstrating the utility of the temporal and spatial control that is provided by these probes.

3) Applying the tandem mass spectrometry technique free-radical initiated peptide sequencing (FRIPS) to complex mixtures of anions.